Complete Analysis of the HIF-1 pathway in C. elegans

Basic statistics

Jupyter Notebook

Predicting interactions between genes

We used correlational analyses to predict genetic interactions. Jupyter Notebook

Transcriptome-wide epistasis analysis

Development of the formalism for, and measurement of, transcriptome-wide epistasis. Jupyter Notebook

Decorrelation within a pathway

Transcriptome weighted correlations between mutants decrease in magnitude the farther apart two genes are from each other. Jupyter Notebook

Non-canonical epistasis

Transcriptomes provide an unbiased view of genetic relationships, which can be used to identify epistasis relationships that are different from what is usually reported. Jupyter Notebook

Enrichment analysis of the hypoxia pathway

We use the WormBase Enrichment Suite to understand the biological functions that the hypoxia pathway is activating in C. elegans. Jupyter Notebook

Finding hif-1-independent responses

Our experimental design allows us to identify genes whose expression is hif-1-independent yet dependent on other genes. In other words, we identify branches in the pathway. Jupyter Notebook

Quality Check

There are known genes that have hif-1-dependent expression. Here, we check that these genes behave as expected from the literature. Jupyter Notebook

Contact us

If there are broken links, or you have questions, you can contact us at: dangeles@caltech.edu

or at

pws@caltech.edu